Chiral separation of several drugs using electrophoresis with dual cyclodextrin systems.

represent high separation efficiencies, rapid analysis time, and low reagent consumption, they have been widely studied as promising analytical methodologies.1–4 Chiral separation is one of the major objectives in CE as well as in chromatographic methods. In these separations, a diverse range of CDs (cyclodextrins) have been employed as chiral additives including nonderivative CDs, derivative neutral CDs, and derivative charged CDs. There is another approach (the dual CD system) that is used to expand the usefulness of CDs for enantioseparations. Recently it was shown that the dual CD system produced better CE enantionseparations.5,6 In a previous paper,7 we have proposed a very simple method to obtain an effective chiral selector (a reaction mixture—a dual CD system—β-CD and β-CD-Glu), described its overall synthetic process and used it to separate two chiral drugs (lobeline and benzhexol). The structure of β-CD-Glu is shown in Fig. 1. In this work, we extend its application using more chiral drugs and further discuss the separation mechanism.